3d if antibodies (Miltenyi Biotec)
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Structured Review
Miltenyi Biotec
3d if antibodies
3d If Antibodies, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3d+if+antibodies/GFP+Antibody/pmc12796729-248-45-49
Average 95 stars, based on 44 article reviews
3d If Antibodies, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3d+if+antibodies/GFP+Antibody/pmc12796729-248-45-49
Average 95 stars, based on 44 article reviews
3d if antibodies - by Bioz Stars,
2026-09
95/100 stars
Images
1) Product Images from "Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexing"
Article Title: Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexing
Journal: STAR Protocols
doi: 10.1016/j.xpro.2025.104296
Figure Legend Snippet: Illustration of MACSima™ Imaging Cyclic Staining (MICS) principle MICS technology was applied (Step 46). (0) Image acquisition of 3D-IF staining in autofluorescence channel, followed by Photobleaching. (2–4) Multi-cyclic imaging: Rounds of 2D-IF staining with FITC, PE and APC coupled antibody fluorochrome-conjugate, image acquisition of respective FITC, PE and APC-channels and signal erasure by photobleaching.
Techniques Used: Imaging, Staining
Figure Legend Snippet: 3D light sheet and 2D multi-cyclic imaging data comparison (Mouse Glioblastoma) (A) Imaris 3D surface rendering of autofluorescence (cyan) and glioblastoma target cells stained with anti-GFP-Alexa Fluor 647 nanobody (red). (B) Imaris 3D surface rendering of autofluorescence (cyan) and glioblastoma target cells stained with anti-GFP-Alexa Fluor 647 nanobody (red) with target plane in yellow. (C) Optical section of target plane of interest. (D) Fluorescence image of physical cryosection. (E) MICS image of section shown in D. (F) MICS image indicating anti-GFP-Alexa Fluor 647 nanobody (red) staining. (G) Magnified merged four color multiparameter MICS image with anti-EGFR (magenta), anti-GFAP (green), anti-NeuN (blue), anti-CD146 (yellow). (H–P) Nine exemplary MICS images with merges of anti-GFP-Alexa Fluor 647 nanobody staining (red) and antibody-conjugates against EGFR (H), Neurofilament (I), Nestin (J), GFAP (K), CD44 (L), CD146 (M), NeuN (N), EphA2 (O) and GLAST (P) (gray) (see “Antibodies”). Scale bars: (A–F) 500 μm; (G) 50 μm; (H–P) 500 μm.
Techniques Used: Imaging, Comparison, Staining, Fluorescence
Figure Legend Snippet: 3D light sheet and 2D multi-cyclic imaging data comparison (Human OvCa) (A) Imaris 3D surface rendering of autofluorescence (cyan) and CD326 positive cells (red). (B) Imaris 3D surface rendering of autofluorescence (cyan) with target plane in yellow. (C) Light sheet guided target plane selection representing CD326 positive cell (purple), CD45 positive cells (red), and CD3 positive cells (green). (D) DAPI overview image of selected tissue slice for 2D MACSima™ imaging. (E) Magnified merged six color multiparameter MICS image with CD45 (green), CD326 (cyan), FOLR1 (purple), Collagen III (red), Collagen IV (red), and CD31 (yellow). (F–L) Single staining MICS images (white) of DAPI (F), CD45 (G), CD326 (H), FOLR1 (I), Collagen III (J), Collagen IV (K), and CD31 (L) (gray) (see “Antibodies”). Scale bars: (A–F) 1 mm; (E) 250 μm; (F–L) 500 μm.
Techniques Used: Imaging, Comparison, Selection, Staining
Related Articles
Labeling:Article Title: Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexing. Article Snippet: .. 15,25 Note: Following steps have been optimized for Alexa Fluor 647 labeled anti-GFP nanobodies (see key resources table) or Article Title: Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexing Article Snippet: .. Timing: 7 days This step describes 3D-IF staining of target cells by passive diffusion at elevated temperatures and defined antibody-conjugate concentrations to improve homogeneous staining within large tissue samples., Note: Following steps have been optimized for Alexa Fluor 647 labeled anti-GFP nanobodies (see ) or Staining:Article Title: Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexing Article Snippet: .. Timing: 7 days This step describes 3D-IF staining of target cells by passive diffusion at elevated temperatures and defined antibody-conjugate concentrations to improve homogeneous staining within large tissue samples., Note: Following steps have been optimized for Alexa Fluor 647 labeled anti-GFP nanobodies (see ) or Diffusion-based Assay:Article Title: Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexing Article Snippet: .. Timing: 7 days This step describes 3D-IF staining of target cells by passive diffusion at elevated temperatures and defined antibody-conjugate concentrations to improve homogeneous staining within large tissue samples., Note: Following steps have been optimized for Alexa Fluor 647 labeled anti-GFP nanobodies (see ) or |